Journal: Frontiers in neuroscience
Article Title: Fluorescence microscopy-based sensitive method to quantify dopaminergic neurodegeneration in a Drosophila model of Parkinson's disease.
doi: 10.3389/fnins.2023.1158858
Figure Lengend Snippet: FIGURE 3 Characterization of DAergic neurodegeneration in the whole fly brain through anti-TH antibody immunostaining and quantification of brain TH protein using western blotting: the image depicts the whole brain mount of the adult Drosophila under control and PQ-treated conditions (A) (CTR, Control; TD, PQ-treated). Quantification of DAergic neurons reveals that the neuronal number remains unaffected (B,C), whereas the fluorescence intensity (of fluorescently labeled secondary antibodies that target primary antibody anti-TH) is significantly decreased in all the clusters (D), and in toto (E) between the control and treated group. The scale bar of the brain images in the panel is 20 μm. (CTR, Control; TD, Treated with 10 mM PQ; Represented images are “merged” Z-stacking images; however, the quantification of DA neuronal number and fluorescence intensity is performed in 3D Z-stack images; PAL, Protocerebral anterior lateral; PPL, Protocerebral posterior lateral; PPM, Protocerebral posterior medial). (F) Stain Free Western Blot analysis shows a reduction of brain TH protein (15%) upon PQ treatment in the fly model of PD [Bio-Rad Stain-Free Western Blotting using total protein normalization method (TPN) (M-protein ladder; CTR-control; PQ- paraquat treated)]. Statistical analysis was performed using a t-test (compared to control). *p < 0.05, **p < 0.01, ***p < 0.001; NS, not-significant.
Article Snippet: The scale bar of the brain images in the panel is 20 μm. (CTR, Control; TD, Treated with 10 mM PQ; Represented images are “merged” Z-stacking images; however, the quantification of DA neuronal number and fluorescence intensity is performed in 3D Z-stack images; PAL, Protocerebral anterior lateral; PPL, Protocerebral posterior lateral; PPM, Protocerebral posterior medial). (F) Stain Free Western Blot analysis shows a reduction of brain TH protein (15%) upon PQ treatment in the fly model of PD [Bio-Rad Stain-Free Western Blotting using total protein normalization method (TPN) (M-protein ladder; CTR-control; PQ- paraquat treated)].
Techniques: Immunostaining, Western Blot, Control, Fluorescence, Labeling, Staining
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![FIGURE 3 Characterization of DAergic neurodegeneration in the whole fly brain through anti-TH antibody immunostaining and quantification of brain TH protein using western blotting: the image depicts the whole brain mount of the adult Drosophila under control and PQ-treated conditions (A) (CTR, Control; TD, PQ-treated). Quantification of DAergic neurons reveals that the neuronal number remains unaffected (B,C), whereas the fluorescence intensity (of fluorescently labeled secondary antibodies that target primary antibody anti-TH) is significantly decreased in all the clusters (D), and in toto (E) between the control and treated group. The scale bar of the brain images in the panel is 20 μm. (CTR, Control; TD, Treated with 10 mM PQ; Represented images are “merged” Z-stacking images; however, the quantification of DA neuronal number and fluorescence intensity is performed in 3D Z-stack images; PAL, Protocerebral anterior lateral; PPL, Protocerebral posterior lateral; PPM, Protocerebral posterior medial). (F) Stain Free Western Blot analysis shows a reduction of brain TH protein (15%) upon PQ treatment in the fly model of PD [Bio-Rad Stain-Free Western Blotting using <t>total</t> <t>protein</t> <t>normalization</t> method <t>(TPN)</t> (M-protein ladder; CTR-control; PQ- paraquat treated)]. Statistical analysis was performed using a t-test (compared to control). *p < 0.05, **p < 0.01, ***p < 0.001; NS, not-significant.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_4762/pm37434762/pm37434762__page7_image1.jpg)